Aqueous two-phase partitioning physical chemistry and bioanalytical applications by Boris Y. Zaslavsky

Cover of: Aqueous two-phase partitioning | Boris Y. Zaslavsky

Published by M. Dekker in New York .

Written in English

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Subjects:

  • Liquid chromatography.,
  • Biomolecules -- Separation.

Edition Notes

Includes bibliographical references and index.

Book details

StatementBoris Y. Zaslavsky.
Classifications
LC ClassificationsQP519.9.L55 Z37 1995
The Physical Object
Paginationxiii, 696 p. :
Number of Pages696
ID Numbers
Open LibraryOL1098172M
ISBN 100824794613
LC Control Number94022868

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Description. Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells.

Papers focus on the theoretical basis and the practical details of the procedures Edition: 1. Description. Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells.

Papers focus on the theoretical basis and the practical details of the procedures used. Aqueous Two-Phase Partitioning: Physical Chemistry and Bioanalytical Applications. 1st Edition. Boris Y. Zaslavsky (Author) › Visit Amazon's Boris Y. Zaslavsky Page. Find all the books, read about the author, and by: Aqueous Two-Phase Systems: Methods and Protocols (Methods in Biotechnology) th Edition.

by Rajni Hatti-Kaul (Editor) ISBN ISBN Why is ISBN important. Partition of Amino Acids and Peptides in Aqueous Two-Phase Systems.

I-Ming Chu, Wen-Yi Chen. Pages Predicting Partition Coefficients of Small Solutes Based on Hydrophobicity.

Mark A. Eiteman. Pages Eukaryotic Cell Partition. James M. Van Alstine. Pages Abstract. Over the last years, aqueous two-phase systems (ATPS) regained an increasing interest due to their potential in the downstream processing of biomolecules. After many years with only a few articles published, a lot of effort and work has been put into studying these systems for the partitioning of a range of compounds including proteins, organic low-molecular weight molecules or metal ions.

Book Reviews Aqueous Two-Phase Partitioning. Physical Chemistry and Bioanalytical Applications; Edited by Boris Y. Zaslavsky, Marcel Dekker, Inc.; New York, ; xiii + pp. $ ISBN 0 3. In a word, this book is exhaustive. Purchase Aqueous Two-Phase Systems, Volume - 1st Edition.

Print Book & E-Book. ISBNFour additional parameters in aqueous two-phase affinity partitioning (ATPAP) that must be considered to optimize the partitioning include (1) pH, (2) ionic strength in polymer/polymer or polymer/salt systems, (3) the nature and concentration of the affinity ligand, and (4) the concentration of modified polymers [23].

The higher the polymer concentrations in the system, the more extreme the partitioning of PEG to the. An Overview of Theoretical Developments for Protein Partitioning in Aqueous Two-Phase Polymer Systems Jesse N. Baskir, T. Alan Hatton Pages Protein Transport Processes in the Water-Water Interface in Incompatible Two Phase Systems.

Aqueous Two-Phase Partition in Biochemical Recovery from Mammalian Cell Culture. Pages Huddleston, Jon G. (et al.). Metal affinity partitioning has been developed to selectively increase the partition coefficients of metal-binding proteins in aqueous two-phase systems (1).

Addition of a small amount of Cu(II)IDA-PEG (Cu(II)-iminodiacetic acid-derivatized polyethyleneglycol) to a two-phase system enhances the partitioning of proteins which contain surface.

Abstract. Phase separation in solutions containing polymer mixtures Aqueous two-phase partitioning book a very common phenomenon; in fact, miscibility of the polymer mixtures is an exception ().Most hydrophilic polymer pairs are incompatible in aqueous solutions yielding two coexisting phases in equilibrium with each other, with each of the phases containing predominantly water and one of the polymer types.

of researchers employ two-phase partitioning techniques in both basic and applied research. Aqueous Two-Phase Systems: Methods and Protocolsis a collection of Aqueous two-phase partitioning book methods intended to provide practical guidelines for those who are new to the area of separations in.

Integrating experimental results and theoretical concepts from a wide range of fields such as drug design, bioseparation, physical organic chemistry, and water-soluble polymers, Aqueous Two-Phase Partitioning describes the phase separation of two polymers in an aqueous medium and the role of the solvent in this phenomenon compares the physicochemical properties of the aqueous polymer phases governing.

Buy Partitioning in Aqueous Two-Phase System: Theory, Methods, Uses, And Applications To Biotechnology on FREE SHIPPING on qualified orders Partitioning in Aqueous Two-Phase System: Theory, Methods, Uses, And Applications To Biotechnology: Harry Walter: : Books.

Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization.

The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined. "synopsis" may belong to another edition of this : Hardcover.

Alternative to conventional methods for enzymes isolation and purification are liquid-liquid extraction methods such as an Aqueous Two-Phase System (ATPS) and Three Phase Partitioning (TPP). The implementation of these methods does not require the availability of expensive equipment or reagents.

Aqueous Two-Phase Partitioning Systems The extraction of an Ab from a crude plant extract can be achieved using aqueous two- phase partitioning [24, 27]. Ab separation by ATPS is achieved by mixing two immiscible reagents, such as poly(ethylene glycol) (PEG), dextran, other polymers, or salts, to form two distinct phases [Balasubramaniam et al., ] [27].

Abstract Protein partitioning in aqueous two-phase systems is a technique that enables one to monitor changes in the 3D structures of proteins in solution resulting from chemical modifications, conformational changes, and interactions with other proteins and ligands.

A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations.

Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by. Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells.

Papers focus on the theoretical basis and the practical details of the Manufacturer: Academic Press. Partitioning in Aqueous Two-Phase Systems: Fundamentals, Applications and Trends. Separation & Purification Reviews: Vol. 45, No. 1, pp. Aqueous Two-Phase Partitioning by Boris Y.

Zaslavsky,available at Book Depository with free delivery worldwide. Aqueous two-phase systems (ATPS) that are formed by mixing a polymer (usually polyethylene glycol, PEG) and a salt (e.g. phosphate, sulphate or citrate) or two polymers and water can be effectively used for the separation and purification of proteins.

The partitioning between both phases is dependen. Title:The Application of Aqueous Two-phase System in the Extraction of Natural Products from Chinese Herbal Medicine: A Review VOLUME: 23 ISSUE: 6 Author(s):Cheng Xiang, Jie Chang*, Ying Ying Yue, Ju Wang and Yan Fu Affiliation:Key Laboratory of Heat Transfer Enhancement and Energy Conservation, Ministry of Education, School of Chemistry and Chemical Engineering, South China University of.

Potential application of aqueous two-phase systems and three-phase partitioning for the recovery of superoxide dismutase from a clarified homogenate of Kluyveromyces marxianus.

Simental-Martínez J(1), Rito-Palomares M, Benavides J. Author information: (1)Centro de Biotecnología FEMSA, Tecnológico de Monterrey, Monterrey, NL,México. A copolymer of 50% ethylene oxide and 50% propylene oxide (EOPO()) has been used in aqueous two‐phase partitioning of amino acids.

This EOPO() copolymer has a cloud point of 70 °C, at which it separates from water. The cloud point can be lowered to 45 °C by the addition of M sodium sulfate.

Sara Ghayour Doozandeh, Gholamreza Pazuki, Ali Asghar, Study of Protein Partitioning in Polymer-Salt Aqueous Two-Phase Systems Using Electrolyte-SAFT (E-SAFT) Equation of State, Journal of Dispersion Science and Technology, /, 33, 5, (), ().

Lorena Capezio, Diana Romanini, Guillermo A. Picó, Bibiana Nerli, Partition of whey milk proteins in aqueous two-phase systems of polyethylene glycol–phosphate as a starting point to isolate proteins expressed in transgenic milk, Journal of Chromatography B, /b,1.

Abstract. The partitioning behavior of norfloxacin (more hydrophilic) and sodium diclofenac (more hydrophobic) was studied considering the use of aqueous two-phase systems.

(ATPS) based in copolymers and dextran. For this research, the phase behavior of systems composed of water, a copolymer (Pluronic PE, Pluronic L35, Pluronic PE, PEG- ran -PPG monobutyl ether and.

Iyyaswami Regupathi, Shanon L. Monteiro, 1-HexylMethylimidazolium Chloride-Potassium Carbonate Aqueous Two Phase System: Equilibrium Characteristics and BSA Partitioning Behavior, Journal of Dispersion Science and Technology, /, 35, 3, (), ().

Partitioning in Aqueous Two-Phase Systems: Fundamentals, Applications and Trends. November ; Separation and Purification Reviews 45(1); Aqueous two phase. PARTITIONING TECHNIQUES -- 5 Single-Step Partitioning in Aqueous Two-Phase Systems -- 6 Partition by Countercurrent Distribution (CCD) -- 7 Liquid\u00E2\u20AC?Liquid Partition Chromatography (LLPC)\/span>\"@ en\/a> ; \u00A0\u00A0\u00A0\n schema:description\/a> \" In Aqueous Two-Phase Systems: Methods and Protocols, Rajni Hatti-Kaul and her.

Aqueous two-phase systems containing self-associating block copolymers. Partitioning of hydrophilic and hydrophobic biomolecules. Svensson M(1), Berggren K, Veide A, Tjerneld F. Author information: (1)Department of Physical Chemistry 1, Lund University, Sweden. Internalized aqueous two-phase separation occurs after addition of TEG to a suspension of droplets containing non-partitioned dextran, resulting in a dextran-enriched sub-compartment and surrounding TEG-loaded coacervate matrix.

(Dextran final concentration, mg mL −1, molecular weight, 70 or k; TEG 6 wt%). Scale bars, 10 μm. 1 INTRODUCTION.

Aqueous two‐phase systems (ATPS) provide mild conditions for the partitioning of thermosensitive and water‐prone organic molecules.

However, at least several of the commonly used phase formers within an ATPS may cause some environmental distress if applied at industrial scale. 5 A better alternative are biodegradable ATPS composed of nontoxic components. Stendahl O, Edebo L, Magnusson KE, Tagesson C, Hjertén S. Surface-charge characteristics of smooth and rough Salmonella typhimurium bacteria determined by aqueous two-phase partitioning and free zones electrophoresis.

Acta Pathol Microbiol Scand B. Oct; 85B (5)–   The volume of fermentation broth processed was mL, the mass of penicillin crystal obtained was g with an average purity of %, the overall recovery ratio of aqueous two-phase partitioning (ATPP) technology was %, and the entire technical process is described.

Partitioning refers to the distribution of solute molecules in the two immiscible phases of a mixture of two solutions, such as an aqueous two-phase system (ATPS).

The partitioning of RNA and peptide has been adjusted in situ to facilitate their assembly into intracellular membraneless organelles. Despite th. It is sure that this pioneering book will help growing interest of the separation scientists in aqueous biphasic systems and broaden the scope of the field.\"--Indian Chemical Society, \/span>\"@ en\/a> ; \u00A0\u00A0\u00A0\n schema:description\/a> \" Metal Ion Separations in Polyethylene Glycol-Based Aqueous Biphasic Systems -- Aqueous.Introduction.

Typical aqueous two-phase systems (ATPS) are usually formed by mixing aqueous solutions of two structurally different polymers or one polymer and one salt above certain concentration (Li et al. ; Walter and Johansson ).Minimizing consumption of organic solvents harmful to the environment ATPS is regarded as a simple and environmentally friendly separation system.Nickel-chelate aqueous two-phase micellar system (ATPMS) was constructed.

• EGFP partitioning behavior in the affinity partitioning system was investigated. • Partition coefficient of EGFP was remarkably enhanced by nickel-chelated Triton X • EGFP was purified directly from cell lysate using the affinity-based ATPMS.

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